Pathology and Microbiology

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INTRODUCTION

Department of Microbiology and Infectious Diseases at Deen Dayal Upadhyay Hospital (DDUH) is well equipped to cater to the needs of outdoor patients and indoor patients.It also caters to patients from other delhi govt hospitals less equipped.Services are also provided at community level during epidemics and pandemics.The rapid response team and infection control team of the department is active during outbreaks.

Regular teaching ,training is conducted in the department for DNB students (postgraduate ),interns,Junior resident,Nursing students,Hospital staff ,MLT students.

Research is promoted in the Department in various fields like Antimicrobial resistance (AMR) surveillance,Public health disease surveillance,Hospital infection control (HIC) surveillance

Department of Microbiology and Infectious Diseases is constituted of various sections:

  • Bacteriology
  • Serology
  • Virology
  • Mycology
  • Immunology
  • Parasitology
  • ICTC
    • Hospital Infection control programme

The Faculty includes senior consultant, specialists, senior residents, medical officers and junior residents,Nursing staff,Counsellors.

Faculty,(Department of Microbiology and Infectious Diseases)

S.No. Name Designation Exchange Number. Landline (Ext.)
1 Dr. Shalini Kakar Specialist Gr-I, HOD Microbiology +91-11-254944402 to 6 512
2. Dr.Anupama Mittal Specialist Gr I,(HOU-1),Infection Control Officer
+91-11-254944402 to 6
512
3. Dr.Poonam Gupta Specialist,(HOU-2),Mycology
+91-11-254944402 to 6
512
4. Dr.Vijeta Maurya Specialist,(HOU-3),Immunology
+91-11-254944402 to 6
512

Sample collection hours- 8.30 AM to 12.30 PM


(Facilities available) – No waiting period

  1. Aerobic Bacterial Culture And Antibiotic Sensitivity Tests
    • Blood
    • Urine
    • Cerebrospinal fluid
    • Respiratory secretions
    • Sputum/ throat swab
    • Other sterile body fluids ( Pleural/ Synovial/ Ascitic/ Vitreous etc)
    • Catheters / Endotracheal Tubes
    • High vaginal swabs
    • Eye/ ear/ Nasal/ Oral swabs
  2. Serology
    • Widal/ Typhidot
    • VDRL (RPR)
    • Hepatitis A/ Hepatitis B/ Hepatitis C/ Hepatitis E
    • HIV in ICTC room near Blood bank
    • Dengue – NS1, Ig M Mac ELISA
    • Chikungunya – Ig M
    • Covid biomarkers- Sr. Ferritin, IL-6, Procalcitonin
  3. Microscopy
    • Direct Grams stain
    • ZN stain for AFB (M. tuberculosis & M leprae) except sputum
    • KOH mount for Fungus
    • Hanging Drop
    • Modified ZN stain for Cryptosporidium Spp.
    • India Ink for Cryptococcus
    • Wet mount for trophozoites
  4. Hospital Infection surveillance
    • Air sampling/ Swabs
    • Tube Test for disinfectants sterility tests

APPENDIX 1

All Media containing vials , bottles, plates may be collected from microbiology daily during routine sample collection hours i.e. 9.00 AM – 11.45 AM and stored at 4-8o C (for max of 3 days after issue)
Serum samples for serology Collected by venipuncture in plain / clot activator vials with acrylic serum separator gel Keep refrigerated at 4-8o C till dispatch to Microbiology lab
Sterile body fluids (e.g. CSF, pericardial, Pleural, Ascitic, Synovial, vitreous, amniotic etc) Collected by site specific puncture. Then inject 2-3 mL in well labelled blood culture bottle+ sterile culture bottle

 

CSF 0.5 mL in BD bottles

 Keep incubated at 35o C till dispatch to Micro Lab
Blood culture. All blood cultures are collected in media containing bottles. Collected by venipuncture in blood culture bottles from atleast 2 different IV sites in two different well labelled bottles. If from central line, then another sample must be taken from another site in a separate blood culture bottles. Keep incubated at 35o C till dispatch to Microbiology Lab
Pus from deep seated abscess Collected by needle and syringe in an empty sterile urine culture vials and 0.5- 2 ml pus in blood culture bottle Keep incubated at 35o C till dispatch to Microbiology Lab
Superficial pus from wounds Collected by touching with swabs moistened with sterile saline and insert in media (Amies & Stuarts) containing test tubes Keep incubated at 35o C till dispatch to Microbiology Lab
Urine Collect either clean catch mid stream urine sample Keep refrigerated at 4-8o C till dispatch to Microbiology lab
Urine Or fresh drops from clamped catheter or suprapubic sample aseptically collected in a sterile culture vials. Rapid transport to Micro Lab
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