Pathology and Microbiology

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INTRODUCTION

Department of Microbiology and Infectious Diseases at Deen Dayal Upadhyay Hospital (DDUH) is situated at ground floor OT Block (Room No M1-M15).It is a tertiary care centre, well equipped to conduct tests required by both outdoor and indoor patients.It also caters to patients refered from other Govt Hospitals in Delhi.Services are also provided at community level during epidemics and pandemics.The Microbiology staff, Rapid Response team and Infection Control team of the department actively participates outside the hospital boundary during community outbreaks.

Research is promoted in the Department in various fields like Antimicrobial resistance (AMR) surveillance, Public health disease surveillance, Hospital infection control (HIC) surveillance

Department of Clinical Microbiology and Infectious Diseases has various sections:

  1. Bacteriology

  2. Serology ( Dengue & Chickunguniya)

  3. Virology ( Hepatitis ( A, B,C & E)

  4. Mycology

  5. Antibiotic senstivity Testing

  6. Parasitology

  1. ICTC ( HIV Counselling & testing centre for DSACS)

  2. Hospital Infection control programme

9. Training Programmes 

      The Department conducts various training programmes in basic Microbiology, Infection Control and Quality protocols

  • For Senior and Junior Doctors
  • For DNB students of all streams
  • For PG students under District Residency Programme 
  • For Medical Lab Technologist of various Health care Institutes
  • For MLT Student
  • For BSc Nursing Students of several institutes
  • For ASHA workers
  • For School students

The Faculty and staff include Senior Specialists, Senior Residents, Nursing staff, Medical Laboratory Technologist and Counsellors.

Faculty,(Department of Microbiology and Infectious Diseases)

S.No.NameDesignationExchange Number.Landline (Ext.)
1Dr. Shalini KakarSpecialist Gr-I, HOD Microbiology+91-11-254944402 to 6512
2.Dr.Anupama MittalSpecialist Gr I Infection Control Officer
+91-11-254944402 to 6
512
3.Dr.Poonam GuptaSpecialist Gr I
+91-11-254944402 to 6
512
4.Dr.Vijeta MauryaSpecialist Gr I
+91-11-254944402 to 6
512

Sample collection hours- 8.30 AM to 12.30 PM


(Facilities available) – No waiting period

  1. Aerobic Bacterial Culture And Antibiotic Sensitivity Tests
    • Blood
    • Urine
    • Cerebrospinal fluid
    • Respiratory secretions
    • Sputum/ throat swab
    • Other sterile body fluids ( Pleural/ Synovial/ Ascitic/ Vitreous etc)
    • Catheters / Endotracheal Tubes
    • High vaginal swabs
    • Eye/ ear/ Nasal/ Oral swabs
  2. Serology
    • Typhidot
    • VDRL (RPR)
    • Hepatitis A/ Hepatitis B/ Hepatitis C/ Hepatitis E
    • HIV in ICTC room near Blood bank
    • Dengue – NS1, Ig M Mac ELISA
    • Chikungunya – Ig M
    • Covid biomarkers- Sr. Ferritin, IL-6, Procalcitonin
  3. Microscopy
    • Direct Grams stain
    • ZN stain for AFB (M. tuberculosis & M leprae) except sputum
    • KOH mount for Fungus
    • Hanging Drop
    • Modified ZN stain for Cryptosporidium Spp.
    • India Ink for Cryptococcus
    • Wet mount for trophozoites
  4. Hospital Infection surveillance
    • Air sampling/ Swabs
    • Tube Test for disinfectants sterility tests

APPENDIX 1

All Media containing vials , bottles, plates may be collected from microbiology daily during routine sample collection hours i.e. 9.00 AM – 11.45 AM and stored at 4-8o C (for max of 3 days after issue)
Serum samples for serologyCollected by venipuncture in plain / clot activator vials with acrylic serum separator gelKeep refrigerated at 4-8o C till dispatch to Microbiology lab
Sterile body fluids (e.g. CSF, pericardial, Pleural, Ascitic, Synovial, vitreous, amniotic etc)

Collected by site specific puncture. Then inject 2-3 mL in well labelled blood culture bottle+ sterile culture bottle

 

CSF 0.5 mL in BD bottles

Keep incubated at 35o C till dispatch to Micro Lab
Blood culture. All blood cultures are collected in media containing bottles.Collected by venipuncture in blood culture bottles from atleast 2 different IV sites in two different well labelled bottles. If from central line, then another sample must be taken from another site in a separate blood culture bottles.Keep incubated at 35o C till dispatch to Microbiology Lab
Pus from deep seated abscessCollected by needle and syringe in an empty sterile urine culture vials and 0.5- 2 ml pus in blood culture bottleKeep incubated at 35o C till dispatch to Microbiology Lab
Superficial pus from woundsCollected by touching with swabs moistened with sterile saline and insert in media (Amies & Stuarts) containing test tubesKeep incubated at 35o C till dispatch to Microbiology Lab
UrineCollect either clean catch mid stream urine sampleKeep refrigerated at 4-8o C till dispatch to Microbiology lab
UrineOr fresh drops from clamped catheter or suprapubic sample aseptically collected in a sterile culture vials.Rapid transport to Micro Lab

List of Investigation and Test Facilities Available

1. Aerobic Bacterial Culture and Identification Tests

 Blood
 Urine
 Cerebrospinal Fluid
 Pus in abscess (drawn with needle Syringe)
 Respiratory Secretions, Sputum / Throat swabs, Other Sterile body fluid (Pleural /
Synovial / Ascitic /Pericardial/ Vitreous etc.)
 Aspirates from Catheters / Endotracheal Tubes,
 High Vaginal swabs
 Eye /Ear/ Nasal / Oral swabs
 Lower conjunctival swabs
 Newborn Umbilical swabs
2. Antibiotic Sensitivity Tests-
 Disc diffusion tests,
 Strip MIC,
 ESBL detection,
 MRSA detection
 MDR detection
 VRE detection

3. Serology

 Widal/Typhidot
 RPR
 Hepatitis B,
 Hepatitis C
 Hepatitis E
 Dengue IgM
 Dengue NS1
 Chikungunya IgM
 HIV in ICTC
4. Microscopy

 Gram’s Stain directly from specimens & culture material
 ZN Stain for AFB (M. tuberculosis & M. leprae in SSS) except sputum
 KOH mount for Fungus
 Hanging Drop for Vibrio cholera in stool
 Modified AFB staining for Cryptosporidium spp
 India Ink for Cryptococcus in CSF
 Albert’s stain for C. diphth

5. Hospital infection Surveillance

 Air surveillance of critical areas (OT& ICU, NICU)
 Bacteriological Testing of Water, Sterility test of blood bags & component Blood bags for
bacteria & fungi
 Sterility test of dialysis fluid & IV fluids for bacteria & fungi
 In use testing of disinfectants
 Environmental samples

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